Busquets was initially ruled out for three weeks after breaking a bone in his foot in the win over Chelsea on March 14.
Going into the game, Roma must be aware that their last trip to Nou Camp ended in a 6-1 defeat.
Messi, 30, started on the bench in Barca’s La Liga clash with Sevilla on Saturday having been forced to sit out both of Argentina’s games during the global break due to injury.
“We’re up against the very best in Messi”, Roma forward Stephan El Shaarawy said. “He is a unique player in history and the world of football”. It’s the quarterfinals of the Champions League and I’ve never played at this stage before.
Luis Suarez, who also struck twice against Roma in that match, was pleased with the team’s attitude against Sevilla.
After a poor 2017-18 season, the Red Devils are plotting to offload most players considered unproductive at the end of this current season.
A full strength squad is expected to be available for Valverde especially after Sergio Busquets returned to training.
Barcelona and Roma will meet in European competition for the fifth time.
The Brazilian has been at Paris Saint-Germain (PSG) since 2013 and has so far failed to win the coveted Champions League despite the Ligue 1 club’s continuous spending over the years.
“There’s a lot of respect, because all three have scored plenty of goals and they’ve also set up many more”, Gundogan told British media.
Just two minutes from full-time and the Messi-inspired side rescued a point. In that period they’ve outscored their opponents 77-12.
Barcelona’s Messi, right, celebrates after scoring against Sevilla during La Liga soccer match between Barcelona and Sevilla at the Sanchez Pizjuan stadium, in Seville, Spain on Saturday, March 31, 2018.
The Champions League match between Barcelona and Roma will be broadcast live on FS1.
After suffering a muscle injury on Saturday against Bologna in the Italian League, Radja Nainggolan has trained with his team, Roma, on Monday and will travel to Barcelona for their Champions League clash.
Roma defender Kostas Manolas praised Lionel Messi in an interview with Il Giornale.